微流體細胞培養系統 Kima Pump

微流體細胞培養系統主要用于細胞在剪切流下進行培養配合Cellix公司微流體芯片可以同時培養8通道內皮細胞。系統的灌注泵可以為細胞培養芯片持續灌注長達48h。詳細信息請!

The KimaTM Pump is a microfluidic pump designed to aid cell culture (e.g. endothelial cells) under physiological conditions (shear flow) in various chips and flow chambers, including Cellix's Vena8 Endothelial+ chips where it is possible to culture 8 cell monolayers simultaneously over 24-48 hours. The KimaTM pump is controlled by the iKimaTM Application for use with the iPod Touch and iPhone. The iPod Touch is included with the sale of the Kima pump. The KimaTM pump is placed inside a standard incubator while the iPod Touch or iPhone, as it's controller, sits on the Universal dock outside the incubator. The KimaTM pump connects easily to Cellix's Vena8 Endothelial+ chip and other manufacturer's flow chambers via fully autoclaveable tubing and delivers pulses of fresh media to cells seeded inside the microchannels.

　　KimaTM Pump with iPod Touch and Controller

　　Kima Pump connected via tubing to Vena8 Endothelial+ chip

　　HUVEC primary cells seeded in Vena8 Endothelial+ chip:

　　1.5hrs post-incubationHUVEC primary cells seeded in Vena8 Endothelial+ chip:

　　72hrs post-perfusion with Kima pump

　　The KimaTM Pump is also applicable across a number of other applications including:

　　· Biofilm culture / Biofilm growthin this case, the Kima pump supports the flow of bacteria through a coated microcapillary enabling bacterial colonization of the surface. Bacteria adhere to the surface, which may be coated with different proteins (e.g. monomannose, trimannose etc.) and subsequently attach to each other forming colonies and causing the film to grow.

　　· Nanoparticle uptake studiesthe Kima pump supports the flow of nanoparticles over cells which have been cultured inside the microchannels of the chips. This set-up can be used to investigate nanoparticle uptake by endothelial cells or other cell types.

　　· Toxicology studiesthe Kima pump supports the flow of compounds over cells which have been cultured inside the microchannels of the chips. This set-up can be used to investigate toxicity of compounds to different cells types over different time periods.

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